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Msc. plant biology

Development of a quantitative molecular tool for the diagnosis of the pathogen Clavibacter michiganensis

In Quebec, greenhouse tomato production is important with more than $60 million in monetary revenue. However, tomatoes are susceptible to many diseases such as bacterial canker, caused by the bacterium Clavibacter michiganensis (Cm). It is one of the most devastating bacterial diseases affecting the tomato industry worldwide. Currently, sanitation practices are the only management strategy available due to the lack of resistant cultivars and effective chemical control methods. Diagnostic methods are particularly necessary to detect the presence of the bacteria and prevent its dispersion. Unfortunately, until now, available quantitative molecular analysis tools detect many false positives and do not differentiate pathogenic from non-pathogenic C. michiganensis. Thus, the project aims to develop a quantitative molecular tool specific to the Cm pathogen. The creation of this tool will help reduce the incidence of Cm thanks to early and accurate detection.

Brochu et al., 2023. Diet and Injection, Important Recommendations to Characterize Clavibacter michiganensis–Tomato Interactions. APS publications. https://doi-org.acces.bibl.ulaval.ca/10.1094/PHP-04-23-0040-RS

PUBLICATIONS

Eichenlaub, R., & Gartemann, K.-H. 2011. The Clavibacter michiganensis Subspecies : Molecular Investigation of Gram-Positive Bacterial Plant Pathogens. Annual Review of Phytopathology, 49(1), 445‑464.

Nandi, M., Macdonald, J., Liu, P., Weselowski, B., Yuan, ZC. 2018. Clavibacter michiganensis ssp. michiganensis: bacterial canker of tomato, molecular interactions and disease management. Mol Plant Pathol.12;19(8):2036–50.

 

Peritore-Galve, F. C., Miller, C., and Smart, C. D. 2023. Characterizing Colonization Patterns of Clavibacter michiganensis During Infection of Tolerant Wild Solanum Species. Phytopathology, 110(3), 574‑581.

 

Thapa, S. P., O’Leary, M., Jacques, M.-A., Gilbertson, R. L., et Coaker, G. 2020. Comparative Genomics to Develop a Specific Multiplex PCR Assay for Detection of Clavibacter michiganensis. Phytopathology, 110(3), 556‑566.

 

Villeneuve, C. & Fortier-Brunelle, K. 2021.Le chancre bactérien de la tomate de champ : biologie et symptômes. MAPAQ.  https://www.agrireseau.net/documents/Document_104024.pdf 

REFERENCES

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